Suppose you have identified a tumor suppressor gene, PTEN, and you believe that loss of this gene will make cancer cells more aggressive and metastatic. How could you use reverse genetics to test your theory?

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Use enhancer trapping to delete the PTEN gene and look for invasive phenotypes.

Use a chimeric gene fusion to generate a gain-of-function PTEN allele and look for the invasive phenotype.

Use RNAi to knock down expression of tumor suppressor genes, and look for cancer cells that become more invasive.

Use a translational fusion protein to knock down expression of PTEN by creating mutant versions of the protein.

 

asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Use RNAi to knock down expression of tumor suppressor genes, and look for cancer cells that become more invasive.

 

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,947 points)

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