You want to use a site-specific recombination system to generate a knockout mouse. If the LoxP sites are oriented as seen here (inverted repeats), what will be the effect on the resulting DNA?

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Intervening DNA is excised.

  

Intervening DNA will be reversed in its orientation.

  

Intervening DNA is duplicated.

  

Intervening DNA will be unaffected because LoxP sites contain inverted rather than direct repeats.

 

asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,941 points)
    

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Intervening DNA will be reversed in its orientation.

 

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,941 points)

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