You want to express GFP in yeast, but after transformation, you realized that you forgot to add a yeast Ori to your expression vector. To your surprise, the yeast are expressing GFP anyway. What is the most likely explanation for this unexpected result?

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Enzymatic degradation of the GFP DNA by nucleases

  

Illegitimate recombination

  

Homologous recombination

  

The E. coli Ori can also function as a yeast Ori in this expression vector.

 

asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Illegitimate recombination

 

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,947 points)

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