Familial fatal insomnia (FFI) is triggered by a GAC → AAC mutation in codon 178 (Asp178Asn) of the PRNP gene. You have an expression vector with the normal human PRNP gene cloned into the MCS. If you want to express the mutant protein in a human cell line, which technique can be used to generate the GAC → AAC mutation?

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ENU mutagenesis


Site-directed mutagenesis


Restriction digests with a specific enzyme


CNBr treatment


asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)

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Site-directed mutagenesis


answered Jun 5, 2013 by GeneX ~Top Expert~ (7,947 points)

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