Human insulin can be expressed in E. coli by generating a construct that fuses the synthetic gene in frame with thelacZ gene, generating a fusion protein. How can you separate insulin from the β-galactosidase peptides to form a functional insulin molecule?

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Incubating the cells with lactose


Treating with transacetylase


Treating with CNBr


Treating with protease to digest the proteins


asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,933 points)

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Treating with CNBr

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,933 points)

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