Human insulin can be expressed in E. coli by generating a construct that fuses the synthetic gene in frame with thelacZ gene, generating a fusion protein. How can you separate insulin from the β-galactosidase peptides to form a functional insulin molecule?

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Incubating the cells with lactose

  

Treating with transacetylase

  

Treating with CNBr

  

Treating with protease to digest the proteins

 

asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Treating with CNBr

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,947 points)

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