While attempting to express a mouse protein in bacteria, you encounter an interesting problem. The mouse protein has many glycine residues, encoded by the codon GGA. When you express the mouse sequence in bacteria, no protein is produced. How might you alter the DNA sequence of the gene to improve protein expression?

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Delete the GGA codons that are preventing proper protein expression.

  

Add a polyadenylation sequence to the end of the DNA sequence.

  

Use site-directed mutagenesis to mutate the GGA codons to AGA codons.

  

Use site-directed mutagenesis to mutate the GGA codons to GGT.

 

asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Use site-directed mutagenesis to mutate the GGA codons to GGT.

 

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,947 points)

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