You want to make zeaxanthin in E. coli, and a friend has offered to give you a plasmid vector containing a gene that encodes one of the necessary enzymes. You can detect the RNA for the gene, but cannot find any protein being translated. Which sequence, required for efficient translation in E. coli, may be missing or mutated in this vector?

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Selectable marker

  

MCS

  

Polyadenylation signal

  

Shine-Dalgarno sequence

 

asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Shine-Dalgarno sequence

 

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,947 points)

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