What is one major advantage of next-generation sequencing over previous Sanger dideoxy sequencing methods?

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Next-generation sequencing can be used to generate longer sequences than dideoxy sequencing, which speeds up the process of sequencing a genome.

Next-generation sequencing relies on chain termination, whereas the Sanger method is based on sequencing “by synthesis” and cannot identify DNA found in small quantities.

 

Next-generation sequencing allows you to generate sequences by combining cloning and PCR, whereas dideoxy sequencing uses cloning alone and cannot identify all regions of DNA if they are expressed at low levels.

 

Next-generation sequencing can be used to sequence trace amounts of DNA by PCR without an additional cloning step, and is high throughput.

 

 

asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Next-generation sequencing can be used to sequence trace amounts of DNA by PCR without an additional cloning step, and is high throughput.

 

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,947 points)

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