You wish to construct a recombinant DNA molecule but cannot identify compatible cohesive ends. Which of the following describes a mechanism for converting a sticky end to a blunt end for recombinant DNA cloning?

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A 3′ overhang can be “chewed back” through the action of a DNA exonuclease.

A 3′ region can be chewed back by DNA exonuclease, which digests the overhanging region so that DNA polymerase can add dNTPs to the 5′ end, producing a double-stranded blunt end.

A 5′ overhang can be used as a binding site for DNA exonuclease, which “chews back” the 5′ overhang and creates a blunt end.

A 5′ overhang can be digested using an additional 5′ restriction endonuclease.

 

asked Jun 5, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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A 3′ overhang can be “chewed back” through the action of a DNA exonuclease.

 

answered Jun 5, 2013 by GeneX ~Top Expert~ (7,947 points)

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