Novobiocin is an inhibitor of DNA gyrase. What effect would you expect to see in bacterial cells treated with novobiocin?

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Cells treated with novobiocin will have excessive amounts of negatively supercoiled DNA, which will cause the DNA to break randomly and kill the bacteria.

Cells treated with novobiocin will accumulate positive supercoiled DNA, as opposed to negative supercoiling, because gyrase induces positive supercoiling in bacteria.

Cells treated with novobiocin will have reduced amounts of supercoiling in their DNA when gyrase is inhibited, so they will have a selective growth advantage and divide more rapidly that untreated bacteria.

Cells treated with novobiocin will be unable to transcribe the DNA because gyrase removes proteins from bacterial DNA and allows regions to be transcribed. Inhibiting gyrase will prevent transcription and ultimately kill the bacteria.

 

asked May 26, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Cells treated with novobiocin will have excessive amounts of negatively supercoiled DNA, which will cause the DNA to break randomly and kill the bacteria.

 

answered May 26, 2013 by GeneX ~Top Expert~ (7,947 points)



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