Southern blot analysis of the three genotypes found at the β-globin locus revealed a distinctive pattern of RFLP variation among the genotypes, while northern blotting reveals no difference. What results would you expect from native electrophoresis of the protein from the three β-globin genotypes?

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A western blot analysis performed on protein from individuals with the three β-globin genotypes looks very similar to the Southern blot results. βAβindividuals have two bands, βSβS individuals have one band, andβAβS individuals have all three bands.

When a western blot analysis is performed on protein from individuals with the three β-globin genotypes,βAβand βSβS individuals each have one distinct band, whereas βAβS individuals have both bands.

A western blot analysis performed on protein from individuals with the three β-globin genotypes shows thatβAβindividuals have a single protein band, whereas βSβS individuals have no protein. βAβS heterozygotes have half as much protein as a homozygous individual.

A western blot analysis performed on protein from individuals with the three β-globin genotypes detects a single protein band, so all genotypes look identical by western blotting.

 

asked May 16, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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When a western blot analysis is performed on protein from individuals with the three β-globin genotypes, βAβA and βSβS individuals each have one distinct band, whereas βAβS individuals have both bands.
answered May 16, 2013 by GeneX ~Top Expert~ (7,947 points)

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