Southern blot analysis of the three genotypes found at the β-globin locus reveals a distinctive pattern of RFLP variation among the genotypes. People who are βAβ A have fragments of 1150 and 200 bp detected by the probe. Knowing the effect of the βS mutation on DdeI sites, what sized fragments would you expect for individuals who areβSβS or βAβS?

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βSβA individuals have a single fragment of 1350 bp; βSβS individuals have two fragments that are 1150 and 1350 bp.

βSβS individuals have two fragments of 200 and 1150 bp; βAβS individuals have three fragments that are 200, 1150, and 1350 bp.

βSβS individuals have a single band of 1350 bp; βAβS individuals have three fragments that are 200, 1150, and 1350 bp.

βSβS individuals have a single fragment of 1350 bp; βAβS individuals have two fragments that are 200 and 1150 bp.

 

asked May 16, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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βSβS individuals have a single band of 1350 bp; βAβS individuals have three fragments that are 200, 1150, and 1350 bp.

 

answered May 16, 2013 by GeneX ~Top Expert~ (7,947 points)

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