Huntington disease is a human trinucleotide repeat disorder. What differences are observed between the fragments of DNA amplified by PCR in individuals with the wild-type versus mutant alleles?

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Wild-type DNA sequences generate shorter PCR fragments than mutant alleles because of different numbers of CAG repeats between the primer binding sites.

Mutant DNA sequences generate shorter PCR fragments than mutant alleles because of different numbers of CAG repeats between the primer binding sites.

Wild-type DNA sequences generate longer PCR fragments than mutant alleles because the mutant has lost one of the primer binding sites due to additional trinucleotide repeats in the mutant.

Wild-type DNA sequences generate shorter PCR fragments than mutant alleles because the mutant has additional primer binding sites located within the extra repeats.

 

asked May 13, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Best answer

 

Wild-type DNA sequences generate shorter PCR fragments than mutant alleles because of different numbers of CAG repeats between the primer binding sites.

 

answered May 13, 2013 by GeneX ~Top Expert~ (7,947 points)

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