You are designing a PCR to allow you to genotype your TERT knockout mice. The primers that you have designed will amplify a 2000 bp fragment if the TERT gene is present, and no band if the gene is absent. Which of the PCR programs would effectively amplify the band?

+1 vote

 

  

95°C for 30 seconds ( 72°C for 30 seconds ( 60°C for 2 minutes

  

95°C for 30 seconds ( 72°C for 2 minutes ( 72°C for 30 seconds

  

95°C for 30 seconds ( 60°C for 30 seconds ( 72°C for 2 minutes

  

72°C for 30 seconds ( 60°C for 30 seconds ( 95°C for 2 minutes

 

asked May 13, 2013 in Genetics by GeneX ~Top Expert~ (7,941 points)
    

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95°C for 30 seconds ( 60°C for 30 seconds ( 72°C for 2 minutes

answered May 13, 2013 by GeneX ~Top Expert~ (7,941 points)

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