DNA polymerase I has a 5’-to-3’ polymerase activity, as well as both 5’-to-3’ and 3’-to-5’ exonuclease activities. Suppose a cell has acquired a mutation that eliminates only the 5’-to-3’ exonuclease activity of DNA polymerase I. What would be the effect on DNA replication?

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The RNA primer will be removed and replaced with DNA, but the newly synthesized DNA cannot be proofread so fidelity of replication will be diminished.

The RNA primer is removed, but cannot be replaced by DNA nucleotides if the 5’-to-3’ exonuclease activity is lost.

Replication would proceed as normal, but proofreading would be slower because it could only proceed 3’ to 5’.

RNA primers required for initiation of replication cannot be removed by DNA polymerase I.

 

asked May 13, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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RNA primers required for initiation of replication cannot be removed by DNA polymerase I.

 

answered May 13, 2013 by GeneX ~Top Expert~ (7,947 points)

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