In PCR, a primer is a short DNA sequence that is complementary to the region of DNA you are amplifying during the reaction. If you select a region of DNA with more guanine and cytosine bases, the primer will anneal more tightly than it would to adenine or thymine bases. Why might that be?

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Three stable phosphodiester bonds form between guanine and cytosine bases, whereas only two phosphodiester bonds form between adenine and thymine.

Three stable hydrogen bonds form between purine bases, whereas only two bonds form between pyrimidines.

Two stable hydrogen bonds form between guanine and cytosine bases, whereas only one bond forms between adenine and thymine.

Three stable hydrogen bonds form between guanine and cytosine bases, whereas only two hydrogen bonds form between adenine and thymine.

 

asked May 13, 2013 in Genetics by GeneX ~Top Expert~ (7,947 points)
    

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Three stable hydrogen bonds form between guanine and cytosine bases, whereas only two hydrogen bonds form between adenine and thymine.

 

answered May 13, 2013 by GeneX ~Top Expert~ (7,947 points)

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