Why is it important to have a high ratio of dNTP nucleotides to ddNTP nucleotides?

+2 votes

 

The ddNTPs are either radioactively or fluorescently labeled, and if too many were present they would saturate the detector.

The extra hydroxyl group of dNTPs makes them noticeably less stable than ddNTPs.

Each ddNTP incorporation will stop synthesis of that strand, and if there are too many ddNTPs present it would be impossible to read a sequence of any significant length.

ddNTPs have a much higher chance of being incorporated into the DNA strand.

 

asked Dec 27, 2012 in Biology by anonymous
    

1 Answer

+1 vote

 

Each ddNTP incorporation will stop synthesis of that strand, and if there are too many ddNTPs present it would be impossible to read a sequence of any significant length.

 

answered Dec 27, 2012 by Ytwsa ~Expert~ (1,092 points)



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