Why are the ddNTP nucleotides used in dideoxy sequencing reactions so useful for determining DNA sequences?

+1 vote

 

  

They pair with the same bases as regular dNTPs.

  

They are modified and thus terminate synthesis whenever they are incorporated into a DNA strand.

  

They are radioactive and can be easily visualized on X-ray film.

  

They have an extra phosphate group, which makes them more likely to be incorporated into a DNA strand.

 

asked Dec 27, 2012 in Biology by anonymous
    

1 Answer

0 votes

 

They are modified and thus terminate synthesis whenever they are incorporated into a DNA strand.

 

answered Dec 27, 2012 by anonymous



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