Which of the following considerations is important when attempting to amplify a gene by PCR?

+1 vote

 

  

finding primers that are complementary to the 3’ to 5’ and the 5’ to 3’ regions on either side of the gene of interest

  

maintaining a constant temperature throughout the PCR reaction

  

including radioactive ddNTPs in the reaction mixture

  

finding primers that are complementary to the 5’ to 3’ strand on either side of the gene of interest

 

asked Dec 27, 2012 in Biology by anonymous
    

1 Answer

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finding primers that are complementary to the 3’ to 5’ and the 5’ to 3’ regions on either side of the gene of interest

 

answered Dec 27, 2012 by anonymous

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