If you screened a cDNA library in E. coli for a growth hormone gene, what would be the best next step after identifying potential colonies with the gene?

+1 vote

 

  

Use restriction enzymes to remove the gene from the plasmid.

  

Sequence the DNA in the bacterial plasmid.

  

Isolate proteins from the colonies to determine if they are synthesizing functional growth hormone.

  

Test to see whether the probe DNA is bound to the colony in question.

 

asked Dec 27, 2012 in Biology by anonymous
    

1 Answer

0 votes

 

Isolate proteins from the colonies to determine if they are synthesizing functional growth hormone.

 

answered Dec 27, 2012 by anonymous

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