Which of the following is the correct sequence of events in cloning a gene into a plasmid?

+1 vote

 

Use ligase to "seal" the inserted gene into the plasmid, identify a restriction site for use in cloning, attach the restriction site to the cDNA ends, and add restriction endonuclease to the plasmid and cDNA.

Add restriction endonuclease to the plasmid and cDNA, use ligase to "seal" the inserted gene into the plasmid, identify a restriction site for use in cloning, and attach the restriction site to the cDNA ends.

Identify a restriction site for use in cloning, add restriction endonuclease to the plasmid and cDNA, attach the restriction site to the cDNA ends, and use ligase to "seal" the inserted gene into the plasmid.

Identify a restriction site for use in cloning, attach the restriction site to the cDNA ends, add restriction endonuclease to the plasmid and cDNA, and use ligase to "seal" the inserted gene into the plasmid.

 

asked Dec 27, 2012 in Biology by anonymous
    

1 Answer

0 votes

 

Identify a restriction site for use in cloning, attach the restriction site to the cDNA ends, add restriction endonuclease to the plasmid and cDNA, and use ligase to "seal" the inserted gene into the plasmid.

 

answered Dec 27, 2012 by anonymous



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