Isolated thylakoids were incubated in an acidic solution at pH 4 until the pH was equilibrated across the thylakoid membrane. The thylakoids were then transferred to a buffer at pH 8 with ADP and inorganic phosphate. ATP was synthesized. Did this experiment require light to generate the ATP?

+1 vote

 

  

Yes, because the hydrogen ion gradient is generated through the transfer of electrons in photosystem II.

  

No, because ATP synthesis depends only on the presence of a hydrogen ion gradient and does not require light directly.

  

Yes, because ATP synthase requires high-energy electrons from photosystem II.

  

No, because ATP synthesis is not part of the light-dependent reactions of photosynthesis.

 

asked Dec 25, 2012 in Biology by anonymous
    

1 Answer

0 votes

 

No, because ATP synthesis depends only on the presence of a hydrogen ion gradient and does not require light directly.

 

answered Dec 25, 2012 by anonymous

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