Refer to this text to answer the following question. To map genes of a bacterial strain, conjugation must be interrupted at given times. Suppose you have Hfr cells of genotype a+b+c+d+e+strR and F= cells of genotype a-b-c-d-e-strS and you combine these two cultures in liquid medium in four blenders at time 0. After intervals of 3, 6, 9, and 12 minutes, you turn on successive blenders. The resulting cultures were then plated on medium containing streptomycin. Why?

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to eliminate all non-conjugated cells

                to eliminate non-conjugated F- cells

                to selectively eliminate cells that have taken in F+ genes

                to eliminate non-conjugating Hfr cells
asked Sep 28, 2014 in Genetics by xxserberusxx ~Rookie~ (113 points)

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