Make predictions of what will happen in each of the lac operon regulation scenarios below

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Draw out each prediction, labeling any cis and trans regulators, inhibitors, inducers, allosteric regulators, and substrates. Illustrate what is happening with RNA Polymerase.

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Scenario 1: Low Glucose, Low Lactose

Scenario 2: High Glucose, High Lactose

Scenario 3: Low Glucose, High Lactose

Scenario 4: E. Coli with the genotype ISO+Z+/F’I+   high levels of glucose and lactose

Scenario 5: 

  1. E. coli with a deleterious mutation at the promoter binding site.  CAP-cAMP complex cannot bind.  Low levels of glucose present, high lactose.  

Scenario 6: 

  1. E. coli with the genotype, I-O+Z+.  Low levels of lactose and high levels of glucose. 

Scenario 7: 

  1. E. coli with the genotype, I-O+Z+/F’I+.  Low levels of lactose and high levels of glucose. 

Scenario 8: 

  1. E. coli with the genotype, I+OcZ+.  Low levels of lactose and low levels of glucose.

Scenario 9: 

  1. E. coli with the genotype, I+OcZ+/ F’O+.  Low levels of lactose and low levels of glucose. 
asked Nov 4, 2013 in Genetics by ktkrahn (45 points)
    

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