In the cell, both plasmids and the chromosome are circular, double stranded molecules. In the laboratory, however, plasmids are often referred to as circular, whereas chromosomal DNA is often referred to as linear. This is because

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    plasmids are supercoiled while the chromosome is not.
     damage during laboratory purification almost always breaks the large chromosomal DNA, whereas plasmid DNA, being smaller, is not broken.
     damage during laboratory purification almost always breaks the small chromosomal DNA, whereas plasmid DNA, being larger, is not broken.
     nucleases often attach the chromosome but not plasmids.
asked May 16, 2012 in Microbiology by anonymous
    

1 Answer

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damage during laboratory purification almost always breaks the large chromosomal DNA, whereas plasmid DNA, being smaller, is not broken.
answered May 17, 2012 by Bioshare ~Top Expert~ (34,070 points)

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